Resin Microscopy and On-Section Immunocytochemistry (Springer Lab Manuals)
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Book Description
Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.
Book Info
Univ. of Wales, Heath Park, Cardiff, UK. Lab manual divided into two main parts: resin embedding, and on-section immunolabeling. Fills the need for a microscopy and immunocytochemistry lab manual that offers the full knowledge of all details of both methodologies. Wire-spiral bound under softcover. DNLM: Immunohistochemistry--methods.
Resin Microscopy and On-Section Immunocytochemistry (Springer Lab Manuals),Geoffrey R. Newman,Jan A. Hobot,Springer,354067277X,Clinical Chemistry,Electron Microscopes & Microscopy,Electron Microscopy In Biology,Electron microscopy,Gums and resins,Histology,Immunochemistry,Immunocytochemistry,Immunohistochemistry,Immunology,Life Sciences - Cytology,Medical,Medical / Nursing,Microscopes & Microscopy,Technique,Science / Biology,crytotechniques,immunolabelling,resin embedding,tissue fixation
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