Editorial Reviews
Book Description
This volume is designed to provide an easily accessible introduction to core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies, such as variations on two-hybrid analysis for investigation of protein-protein interactions or investigation of protein-nucleic acid interactions.
Book Info
Auburn Univ., AL. Designed to provide an introduction to core strategies in the application of modern recombinant DNA technologies. For experienced scientists and graduate students. Wire-spiral binding.
Genetic Library Construction and Screening: Advanced Techniques and Applications (Springer Lab Manuals),R.C. Bird,Bruce F. Smith,Springer,3540672788,Dna (Deoxyribonucleic Acid),Genetics,Laboratory Medicine,Laboratory manuals,Life Sciences - Biochemistry,Life Sciences - Biology - General,Life Sciences - Cytology,Life Sciences - Genetics & Genomics,Medical,Medical / Nursing,Molecular cloning,Polymerase Chain Reaction,Science,2-Hybrid,Biochemistry,Cellular biology,DNA,Gene Cloning,Gene Library,Genetic engineering,Hybridization,Medical research,Molecular Biology,PCR,Science / Biology,cDNA
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